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Quantitative
Real-Time PCR by Mx4000
from
Stratagene
Quantitative
real-time PCR is a highly sensitive and specific fluorescence-based
technique that measures the production of a target amplicon
during the exponential phase of the polymerase chain reaction
when PCR efficiency is least influenced by reaction variables.
The
use of fluorogenic probes reduces the time to obtain results
by eliminating post-PCR processing. Relative quantitation,
based on expression of an internal housekeeping control, as
well as absolute quantitation, based on standard curves of
the target amplicon, can be achieved using real-time PCR methodology.
Real-time QPCR has many other applications including allelic
discrimination, viral load, cDNA microarray validation, gene
dosage and SNP analyses.
The
benefits of the automated MX4000 technology include real-time
PCR detection; closed-tube assay, no electrophoresis, highly
reproducible quantitative results, large dynamic range, 96
sample throughput and ability to analyse results during the
reaction.
| The
Mx4000 for Real-time PCR
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4
Photomultipliers for Multiplexing
96
well format 20-50 ul vol. |
The
Optics
Applications
- DNA
target quantification
- Gene
expression
- SNP
detection, Allele discrimination, GenotypingDNA methylation,
Apoptosis
- Viral
load assays, Pathogen detection
- Clinical
diagnosis (Cancer therapy response)
Visit
Stratagene's website for more information about the MX4000
real-time PCR system. Photos and charts shown courtesy of Stratagene.
Other
Useful Links
Laboratory
Tools at Stratagene
Design
Primers for free with Primer3
BLAST
site for checking sequences
MX4000
Applications
Cepheid
QPCR links
IDTDNA
QPCR links
Biosearchtech
QPCR links
Oligo
Houses
Qiagen/Operon
Sigma/Genosys
Oligos
Etc.
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