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Laser
Capture Microdissection
LCM
offers a method for procuring pure cells from specific microscopic
regions of tissue sections and which allows the application
of molecular analysis methods to study evolving disease lesions
in actual tissue.
The
benefits of the lab's AutoPix LCM are multiple slide handling,
versatile capture modes (manual, semimanual and automated),
rapid capture of large numbers of cells, image inventory software,
very easy software controls, robust instrumentation and robotics
and low energy infrared laser that does not damage cells.
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Automated,
versatile
and
self-contained
Laser Capture Microdissection (LCM) system
The
completely enclosed instrument
minimizes
contamination and sensitively detects fluorescent samples.
The
high-intensity fluorescence illuminator has three filer
cubes for red, green and blue
fluorescence.
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The
Sample Station
Three
different sample slides can be dissected at a single
session. The cap tray holds eight caps, either HS or
Macro. Used caps containing captured cells are robotically
moved to the QC station for evaluation and then to the
removeable unload station for retrieval. DNA, RNA or
protein is then extracted using reagent kits available
from Arcturus. |
Visit
the Arcturus
web site for more information about the Arcturus
AutoPix Laser Capture Microdissection system. This web
site has all
of the information you need to get started. Please read the
two application notes before contacting us to use the instrument.
Photos and charts shown courtesy of Arcturus.
Laser
Capture Microdissection (LCM) Systems
Application
Notes
Tissue
Scrape Protocol for Verifying RNA Quality Using the PicoPure™
RNA Isolation Kit
Optimized
Protocol for Preparing and Staining LCM Samples from Frozen
Tissue and Extraction of High-Quality RNA
Supplies
You Will Need
CapSure
Caps for LCM
PicoPure™
RNA Isolation Kit
RiboAmp®
OA RNA Amplification Kit
HistoGene™
LCM Frozen Section Staining Kit
Other
Useful Links
LCM
at NICHD
LCM
at NIEHS
NCI/CIT
Molecular Profiling Initiative
Useful
Bibilography
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