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Immunohistochemistry
Specialty Testing
The
Molecular Core laboratory's Ventana
Discovery is a totally automated staining module that
uses bar code technology. The Discoveryoffers walk-away automation,
reliable reproducibility, flexibility and data management,
as well as total automation in the areas of IHC staining of
frozen or paraffin embedded sections of animal or human tissue,
FISH and ISH using DNA, RNA or oligonucleotide probes on sections
or microarrays (gene expression).
Immunohistochemistry
- Non-solvent
based removal of paraffin
- Automated
epitope unmasking
- Flexible
primary antibody incubation options
- Four
available detection chemistries
- DAB,
AEC, Fast Red, NBT
- Automated
counterstaining
In
Situ Hybridization
- Rehydration
of slides
- Promote
uniform spreading/reduce nonspecific hybridation
- Denaturation
- Hybridization
- Stringency
washes
- Optional
secondary detection
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DNA
Microarray
- Target
unmasking
- Automated
probe application
- Selectable
time and temperature denaturation
- Selectable
time and temperature hybridization
- Selectable
time, stringency and temperature washes
- Detection
counterstain
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EOS
DNA Microarray |
Photos
and charts shown courtesy of Ventana Discovery.
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